AF 647 Alkyne (Alexa Fluor 647 Alkyne analog) is a water-soluble, bright, far-red-fluorescent dye with excitation ideally suited for the 633 nm or 647 nm laser lines. A significant advantage to using long wavelength dyes such as Cy5 or AFDye™ 647 dye over other fluorophores is the low autofluorescence of biological specimens in this region of the spectrum.
AFDye™ 647 Alkyne is a very bright, far-red-fluorescent, pH insensitive alkyne-activated probe routinely used for imaging of azide-containing biomolecules. AFDye™ 647 Alkyne reacts with azides via a copper-catalyzed click reaction (CuAAC) to form a stable triazole linker. A probe for copper-less detection (AFDye™ 647 DBCO) is also available for application where the presence of copper is not acceptable.
AFDye™ 647 is structurally similar to Alexa Fluor® 647, and spectrally is almost identical to Cy5 Dye, Alexa Fluor® 647, CF® 647 Dye, or any other Cyanine5 based fluorescent dyes.