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AFDye 647 Alkyne

Catalog#
Unit Size
Price (USD)
Availability
Qty
Cat.#: 1126-1
Unit size: 1 mg
$149.00
in stock
Cat.#: 1126-5
Unit size: 5 mg
$465.00
in stock
Cat.#: 1126-25
Unit size: 25 mg
$1,495.00
in stock

AF 647 Alkyne (Alexa Fluor 647 Alkyne analog) is a water-soluble, bright, far-red fluorescent dye with excitation ideally suited for the 633 nm or 647 nm laser lines. A significant advantage to using long wavelength dyes such as Cy5 or AFDye™ 647 dye over other fluorophores is the low autofluorescence of biological specimens in this region of the spectrum.

AFDye™ 647 Alkyne is a bright, far-red fluorescent, pH insensitive alkyne-activated probe routinely used for imaging of azide-containing biomolecules. AFDye™ 647 Alkyne reacts with azides via a copper-catalyzed click reaction (CuAAC) to form a stable triazole linker. A probe for copper-less detection (AFDye™ 647 DBCO) is also available for application where the presence of copper is not acceptable.

AFDye™ 647 is structurally similar to Alexa Fluor® 647, and spectrally is almost identical to Cy5 Dye, Alexa Fluor® 647, CF® 647 Dye, or any other Cyanine5 based fluorescent dyes.

Reactivity
Azide, picolyl azide
Abs/Em Maxima
648/671 nm
Extinction coefficient
250,000 cm-1M-1
Solubility
Water, DMSO, DMF
Spectrally similar dyes
Alexa Fluor® 647, DyLight® 649,
Molecular weight
910.10
Storage conditions
-20C
Shipping conditions
Ambient temperature

spectrum