AFDye 594 Picolyl Azide

Catalog#: 1108-1
Unit size: 1 mg
In stock
Catalog#: 1108-5
Unit size: 5 mg
In stock
Catalog#: 1108-25
Unit size: 25 mg
In stock
Catalog#: 1108-100
Unit size: 100 mg
In stock

AFDye™ 594 Picolyl Azide (also known as Alexa Fluor 594 Picolyl Azide) is an advanced fluorescent probe that incorporates a copper-chelating motif to raise the effective concentration of Cu(I) at the reaction site. The rate of CuAAC reaction depends on concentrations of all reagents, including copper, thus the raise of the effective copper concentration at the reaction site dramatically increase the rate of CuAAC reaction without the need to increase concentration of azide reagent and copper.

In addition, the use of AFDye™ 594 Picolyl Azide instead of conventional AFDye™ 594 Azide allows for at least a tenfold reduction in the concentration of the copper catalyst without sacrificing the efficiency of labeling.

In summary, the introduction of a picolyl moiety into an azide probe leads to a substantial increase in the sensitivity of alkyne detection. This will be of special value for the detection of low abundance targets or where significant increase in signal intensity is desired.

AFDye™ 594 dye structurally is identical to Invitrogen’s Alexa Fluor® 594

Alkyne, cyclooctyne
Abs/Em Maxima
590/617 nm
Extinction coefficient
92,000 cm-1M-1
Water, DMSO, DMF
Spectrally similar dyes
Alexa Fluor® 594, CF™ 594, DyLight® 594
Molecular weight
Storage conditions
Shipping conditions
Ambient temperature

Alexa Fluor® Dyes is a registered trademark of Invitrogen, part of Thermo Fisher Scientific. Fluoroprobes is not affiliated with or endorsed by Thermo Fisher Scientific. AFDyes are GENERIC versions of Alexa Fluor® Dyes and ARE NOT manufactures, distributed, or affiliated in any way with Thermo Fisher Scientific.