AF 594 Maleimide

Catalog#: 1102-1
Unit size: 1 mg
$129.00
In stock
Catalog#: 1102-5
Unit size: 5 mg
$395.00
In stock
Catalog#: 1102-25
Unit size: 25 mg
$1,395.00
In stock
Catalog#: 1102-100
Unit size: 100 mg
$2,895.00
In stock

AF™ 594 (Alexa Fluor 594 Maleimide Dye) is bright, water-soluble, and pH-insensitive from pH 4 to pH 10 red-fluorescent dye with absorption and emission maxima at 590 and 617 nm, respectively. It can be used with the 561 nm and 594 nm laser lines. AF™ 594 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates often used for generation of stable signal in imaging and flow cytometry. The brightness and photostability of this dye are best suited to direct imaging of low-abundance targets.

Maleimide is the most popular sulfhydryl-reactive reactive group for conjugating the dye to a thiol group on a protein, oligonucleotide thiophosphate, or low molecular weight ligand. The maleimide group specifically and efficiently reacts with reduced thiols (sulfhydryl groups, –SH) at pH 6.5 to 7.5 to form stable thioether bond. The resulting conjugates exhibit brighter fluorescence and greater photostability than the conjugates of many other spectrally similar fluorophores.

AF™ 594 dye structurally is identical to Invitrogen’s Alexa Fluor® 594

Reactivity
Thiol (Cys)
Abs/Em Maxima
588/612 nm
Extinction coefficient
96,000 cm-1M-1
Solubility
Water, DMSO, DMF
Spectrally similar dyes
Alexa Fluor® 594, CF® 594, Texas Red
Molecular weight
908.97
Storage conditions
-20C
Shipping conditions
Ambient temperature

Enter only one — either volume or amount of protein to be labeled
Enter only one — either maximum concentration of DMSO or volume DMSO added to label

Alexa Fluor® Dyes is a registered trademark of Invitrogen, part of Thermo Fisher Scientific. Fluoroprobes is not affiliated with or endorsed by Thermo Fisher Scientific. AF Dyes are GENERIC versions of Alexa Fluor® Dyes and ARE NOT manufactures, distributed, or affiliated in any way with Thermo Fisher Scientific.