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AFDye 594 C5 Maleimide

Catalog#
Unit Size
Price (USD)
Availability
Qty
Cat.#: 1102-1
Unit size: 1 mg
$139.00
in stock
Cat.#: 1102-5
Unit size: 5 mg
$445.00
in stock
Cat.#: 1102-25
Unit size: 25 mg
$1,395.00
in stock
Cat.#: 1102-100
Unit size: 100 mg
$2,895.00
in stock

AFDye 594 C5 Maleimide (Alexa Fluor® 594 C5 Maleimide equivalent) is bright, water-soluble, and pH-insensitive from pH 4 to pH 10 red-fluorescent dye with absorption and emission maxima at 590 and 617 nm, respectively. It can be used with the 561 nm and 594 nm laser lines. AFDye 594 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates often used for generation of stable signal in imaging and flow cytometry. The brightness and photostability of this dye are best suited to direct imaging of low-abundance targets.

Maleimide is the most popular sulfhydryl-reactive reactive group for conjugating the dye to a thiol group on a protein, oligonucleotide thiophosphate, or low molecular weight ligand. The maleimide group specifically and efficiently reacts with reduced thiols (sulfhydryl groups, –SH) at pH 6.5 to 7.5 to form stable thioether bond. The resulting conjugates exhibit brighter fluorescence and greater photostability than the conjugates of many other spectrally similar fluorophores.

AFDye 594 C5 Maleimdie dye structurally is identical to Alexa Fluor® 594 C5 Maleimide.

Reactivity
Thiol (Cys)
Abs/Em Maxima
590/617 nm
Extinction coefficient
96,000 cm-1M-1
Solubility
Water, DMSO, DMF
Spectrally similar dyes
Alexa Fluor® 594, CF® 594, Texas Red
Molecular weight
885.98
Storage conditions
-20C
Shipping conditions
Ambient temperature

spectrum

Enter only one — either volume or amount of protein to be labeled
Enter only one — either maximum concentration of DMSO or volume DMSO added to label

Alexa Fluor® Dyes is a registered trademark of Thermo Fisher Scientific.