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AFDye 594 Azide

Catalog#
Unit Size
Price (USD)
Availability
Qty
Cat.#: 1104-1
Unit size: 1 mg
$149.00
in stock
Cat.#: 1104-5
Unit size: 5 mg
$445.00
in stock
Cat.#: 1104-25
Unit size: 25 mg
$1,395.00
in stock

AFDye™ 594 Azide (also known as Alexa Fluor® 594 Azide) is a bright, red-fluorescent azide-activated probe that reacts with terminal alkynes via a copper-catalyzed click reaction (CuAAC). It also reacts with strained cyclooctyne via a copper-free click chemistry reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures.

AFDye™ 594 is bright, water-soluble, and pH-insensitive from pH 4 to pH 10 red-fluorescent dye with absorption and emission maxima at 590 and 617 nm, respectively. It can be used with the 561 nm and 594 nm laser lines. AFDye™ 594 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates often used for generation of stable signal in imaging and flow cytometry.

AFDye™ 594 Azide structurally is identical to Invitrogen’s Alexa Fluor® 594 Azide

Reactivity
Alkyne, cyclooctyne
Abs/Em Maxima
590/617 nm
Extinction coefficient
92,000 cm-1M-1
Solubility
Water, DMSO, DMF
Spectrally similar dyes
Alexa Fluor® 594, CF™ 594, DyLight® 594
Molecular weight
948.16
Storage conditions
-20C
Shipping conditions
Ambient temperature

spectrum
Alexa Fluor® Dyes is a registered trademark of Thermo Fisher Scientific.