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AFDye 594 Alkyne

Catalog#
Unit Size
Price (USD)
Availability
Qty
Cat.#: 1106-1
Unit size: 1 mg
$149.00
in stock
Cat.#: 1106-5
Unit size: 5 mg
$445.00
in stock
Cat.#: 1106-25
Unit size: 25 mg
$1,395.00
in stock

AFDye™ 594 Alkyne (Alexa Fluor® 594 Alkyne equivalent) is a bright, red-fluorescent alkyne-activated probe routinely used for imaging of azide-containing biomolecules. AFDye™ 594 Alkyne reacts with azides via a copper-catalyzed click reaction (CuAAC) to form a stable triazole linker. A probe for copper-less detection, AFDye™ 594 DBCO is also available for application where the presence of copper is not acceptable.

AFDye™ 594 is bright, water-soluble, and pH-insensitive from pH 4 to pH 10 red-fluorescent dye with absorption and emission maxima at 590 and 617 nm, respectively. It can be used with the 561 nm and 594 nm laser lines. AFDye™ 594 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates often used for generation of stable signal in imaging and flow cytometry.

Reactivity
Azide
Abs/Em Maxima
590/617 nm
Extinction coefficient
92,000 cm-1M-1
Solubility
Water, DMSO, DMF
Spectrally similar dyes
Alexa Fluor® 594, CF™ 594, DyLight® 594
Molecular weight
861.04
Storage conditions
-20C
Shipping conditions
Ambient temperature

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