AFDye 568 NHS Ester

Catalog#: 1081-1
Unit size: 1 mg
$129.00
In stock
Catalog#: 1081-5
Unit size: 5 mg
$395.00
In stock
Catalog#: 1081-25
Unit size: 25 mg
$1,295.00
In stock
Catalog#: 1081-100
Unit size: 100 mg
$2,495.00
In stock

AFDye™ 568 NHS Ester (Alexa Fluor 568 NHS Ester equivalent) is an amine-reactive, orange fluorescent dye routinely used to label proteins or antibodies through the primary amines (Lys), amine-modified oligonucleotides, and other amine-containing biomolecules. AFDye™ 568 dye can be used for proteins labeling at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection.

AFDye™ 568 is a water-soluble, photostable, and bright orange dye with pH insensitive emission from pH 4 to pH 10. The excitation of AFDye™ 568 dye ideally suited for the 568 nm laser line on the Ar-Kr mixed-gas laser. AF™ 568 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates often used for generation of stable signal in imaging and flow cytometry.

AFDye™ 568 NHS Ester is structurally identical to Alexa Fluor® 568 NHS Ester sold by ThermoFisher Scientific.

Reactivity
Primary amine
Abs/Em Maxima
578/602 nm
Extinction coefficient
88,000 cm-1M-1
Solubility
Water, DMSO, DMF
Spectrally similar dyes
Alexa Fluor® 568, CF™ 568
Molecular weight
791.80 (protonated)
Storage conditions
-20C
Shipping conditions
Ambient temperature

Enter only one — either volume or amount of protein to be labeled
Enter only one — either maximum concentration of DMSO or volume DMSO added to label

Alexa Fluor® Dyes is a registered trademark of Invitrogen, part of Thermo Fisher Scientific. Fluoroprobes is not affiliated with or endorsed by Thermo Fisher Scientific. AFDyes are GENERIC versions of Alexa Fluor® Dyes and ARE NOT manufactures, distributed, or affiliated in any way with Thermo Fisher Scientific.