AFDye™ 568 NHS Ester (Alexa Fluor 568 NHS Ester equivalent) is an amine-reactive, orange fluorescent dye routinely used to label proteins or antibodies through the primary amines (Lys), amine-modified oligonucleotides, and other amine-containing biomolecules. AFDye™ 568 dye can be used for proteins labeling at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection.
AFDye™ 568 is a water-soluble, photostable, and bright orange dye with pH insensitive emission from pH 4 to pH 10. The excitation of AFDye™ 568 dye ideally suited for the 568 nm laser line on the Ar-Kr mixed-gas laser. AF™ 568 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates often used for generation of stable signal in imaging and flow cytometry.
AFDye™ 568 NHS Ester is structurally identical to Alexa Fluor® 568 NHS Ester sold by ThermoFisher Scientific.