AFDye 568 Maleimide

Catalog#: 1082-1
Unit size: 1 mg
$129.00
In stock
Catalog#: 1082-5
Unit size: 5 mg
$395.00
In stock
Catalog#: 1082-25
Unit size: 25 mg
$1,295.00
In stock
Catalog#: 1082-100
Unit size: 100 mg
$2,495.00
In stock

AFDye™ 568 Maleimide (Alexa Fluor® 568 C5 Maleimide equivalent) is a bright, and highly photostable, orange-fluorescent probe optimally excited by the 568 nm laser line on the Ar-Kr mixed-gas laser. AFDye™ 568 Maleimide is water-soluble and its fluorescence is pH independent over a wide pH range. The brightness and photostability of this dye are best suited to direct imaging of low-abundance targets.

Maleimide is the most popular sulfhydryl-reactive reactive group for conjugating the dye to a thiol group on a protein, oligonucleotide thiophosphate, or low molecular weight ligand. The maleimide group specifically and efficiently reacts with reduced thiols (sulfhydryl groups, –SH) at pH 6.5 to 7.5 to form stable thioether bond. The resulting conjugates exhibit brighter fluorescence and greater photostability than the conjugates of many other spectrally similar fluorophores.

AFDye™ 568 Maleimide is structurally identical to Invitrogen’s Alexa Fluor® 568 C5 Maleimide

Reactivity
Primary amine
Abs/Em Maxima
575/600 nm
Extinction coefficient
92,000 cm-1M-1
Solubility
Water, DMSO, DMF
Spectrally similar dyes
Alexa Fluor® 568, CF™ 568
Molecular weight
857.93
Storage conditions
-20C
Shipping conditions
Ambient temperature

Enter only one — either volume or amount of protein to be labeled
Enter only one — either maximum concentration of DMSO or volume DMSO added to label

Alexa Fluor® Dyes is a registered trademark of Invitrogen, part of Thermo Fisher Scientific. Fluoroprobes is not affiliated with or endorsed by Thermo Fisher Scientific. AFDyes are GENERIC versions of Alexa Fluor® Dyes and ARE NOT manufactured, distributed, or affiliated in any way with Thermo Fisher Scientific.