AFDye™ 568 DBCO (Alexa Fluor® 568 DBCO) reacts with azides via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. In application where the presence of copper is a concern Alexa Fluor 568 DBCO is an ideal alternative to copper requiring fluorescent alkynes.
AFDye™ 568 (also known as Alexa Fluor® 568) is a bright, and highly photostable, orange-fluorescent probe optimally excited by the 568 nm laser line on the Ar-Kr mixed-gas laser. This probe is water-soluble and its fluorescence is pH independent over a wide pH range. The brightness and photostability of this dye are best suited to direct imaging of low-abundance targets.
AFDye™ 568 structurally is identical to Alexa Fluor® 568 dye sold by ThermoFisher Scientific.