AFDye™ 568 Alkyne (Alexa Fluor 568 Alkyne equivalent) is a bright, orange-fluorescent alkyne-activated probe routinely used for imaging of azide-containing biomolecules. AF™ 568 Alkyne reacts with azides via a copper-catalyzed click reaction (CuAAC) to form a stable triazole linker. A probe for copper-less detection, AFDye™ 568 DBCO is also available for application where the presence of copper is not acceptable.
AFDye™ 568 is a bright, and highly photostable, orange-fluorescent probe optimally excited by the 568 nm nm laser line on the Ar-Kr mixed-gas laser. This probe is water-soluble and its fluorescence is pH independent over a wide pH range. The brightness and photostability of this dye are best suited to direct imaging of low-abundance targets.
AFDye™ 568 structurally is identical to Alexa Fluor® 568 dye sold by ThermoFisher Scientific.