AFDye 546 NHS ester

Catalog#: 1051-1
Unit size: 1 mg
In stock
Catalog#: 1051-5
Unit size: 5 mg
In stock
Catalog#: 1051-25
Unit size: 25 mg
In stock
Catalog#: 1051-100
Unit size: 100 mg
In stock

AFDye™ 546 NHS Ester (Alexa Fluor 546 NHS Ester) is the most popular tool for modifying proteins or antibodies through the primary amines (Lys), amine-modified oligonucleotides, and other amine-containing biomolecules with AFDye™ 546 label. The labeling occurs most efficiently at pH 7-9 and forms a stable, covalent amide bond.

AFDye™ 546 is water-soluble, and pH-insensitive from pH 4 to pH 10 orange-fluorescent dye with absorption and emission maxima at 554 and 570 nm, respectively. It can be used with the 488 nm, 532 nm and 543 laser lines. AF™ 546 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates often used for generation of stable signal in imaging and flow cytometry.

AFDye™ 546 dye structurally is identical to Invitrogen’s Alexa Fluor® 546 NHS Ester

Primary amine
Abs/Em Maxima
554/570 nm
Extinction coefficient
112,000 cm-1M-1
Water, DMSO, DMF
Spectrally similar dyes
Alexa Fluor® 546, TAMRA, CF™ 543, MB™ 543
Molecular weight
Storage conditions
Shipping conditions
Ambient temperature

Enter only one — either volume or amount of protein to be labeled
Enter only one — either maximum concentration of DMSO or volume DMSO added to label

Alexa Fluor® Dyes is a registered trademark of Invitrogen, part of Thermo Fisher Scientific. Fluoroprobes is not affiliated with or endorsed by Thermo Fisher Scientific. AFDyes are GENERIC versions of Alexa Fluor® Dyes and ARE NOT manufactures, distributed, or affiliated in any way with Thermo Fisher Scientific.