AFDye 546 DBCO

Catalog#: 1057-1
Unit size: 1 mg
In stock
Catalog#: 1057-5
Unit size: 5 mg
In stock
Catalog#: 1057-25
Unit size: 25 mg
In stock
Catalog#: 1057-100
Unit size: 100 mg
In stock

AFDye™ 546 DBCO an azide reactive probe that can be used for imaging azide-tagged biomolecules via a copper-free “click reaction”. DBCO moiety reacts with azides to form a stable triazole and does not require Cu-catalyst or elevated temperatures. In application where the presence of copper is a concern AFDye™ 546 DBCO is an ideal alternative to copper requiring fluorescent alkynes.

AFDye™ 546 is water-soluble, and pH-insensitive from pH 4 to pH 10 orange-fluorescent dye with absorption and emission maxima at 554 and 570 nm, respectively. It can be used with the 488 nm and 532 nm laser lines. AFDye™ 546 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates often used for generation of stable signal in imaging and flow cytometry.

AFDye™ 546 dye structurally is identical to Alexa Fluor® 546 dye sold by ThermoFisher Scientific.

Abs/Em Maxima
554/570 nm
Extinction coefficient
112,000 cm-1M-1
Water, DMSO, DMF, MeOH
Spectrally similar dyes
Alexa Fluor® 546, TAMRA, CF™ 543, MB™ 543
Molecular weight
Storage conditions
Shipping conditions
Ambient temperature

Alexa Fluor® Dyes is a registered trademark of Invitrogen, part of Thermo Fisher Scientific. Fluoroprobes is not affiliated with or endorsed by Thermo Fisher Scientific. AFDyes are GENERIC versions of Alexa Fluor® Dyes and ARE NOT manufactures, distributed, or affiliated in any way with Thermo Fisher Scientific.