AFDye™ 546 DBCO is an azide-reactive probe that can be used for imaging azide-tagged biomolecules via a copper-free click reaction. The DBCO moiety reacts with azides to form a stable triazole and does not require a Cu-catalyst or elevated temperatures. In applications where the presence of copper is a concern, AFDye™ 546 DBCO is an ideal alternative to copper-requiring fluorescent alkynes.
AFDye™ 546 is a water-soluble, pH-insensitive (from pH 4-10), orange-fluorescent dye with absorption and emission maxima at 554 and 570 nm, respectively. It can be used with the 488 nm and 532 nm laser lines. AFDye™ 546 dye conjugates to a variety of antibodies, peptides, proteins, tracers, and amplification substrates, which are often used for generating stable signals in imaging and flow cytometry.
AFDye™ 546 dye is structurally identical to Alexa Fluor® 546 dye sold by Thermo Fisher Scientific.