• SIGN IN
  • No products in the cart.

AFDye 546 DBCO

Catalog#
Unit Size
Price (USD)
Availability
Qty
Cat.#: 1057-1
Unit size: 1 mg
$149.00
in stock
Cat.#: 1057-5
Unit size: 5 mg
$485.00
in stock
Cat.#: 1057-25
Unit size: 25 mg
$1,495.00
in stock

AFDye™ 546 DBCO is an azide-reactive probe that can be used for imaging azide-tagged biomolecules via a copper-free click reaction. The DBCO moiety reacts with azides to form a stable triazole and does not require a Cu-catalyst or elevated temperatures. In applications where the presence of copper is a concern, AFDye™ 546 DBCO is an ideal alternative to copper-requiring fluorescent alkynes.

AFDye™ 546 is a water-soluble, pH-insensitive (from pH 4-10), orange-fluorescent dye with absorption and emission maxima at 554 and 570 nm, respectively. It can be used with the 488 nm and 532 nm laser lines. AFDye™ 546 dye conjugates to a variety of antibodies, peptides, proteins, tracers, and amplification substrates, which are often used for generating stable signals in imaging and flow cytometry.

AFDye™ 546 dye is structurally identical to Alexa Fluor® 546 dye sold by Thermo Fisher Scientific.

Reactivity
Azides
Abs/Em Maxima
554/570 nm
Extinction coefficient
112,000 cm-1M-1
Solubility
Water, DMSO, DMF, MeOH
Spectrally similar dyes
Alexa Fluor® 546, TAMRA, CF™ 543, MB™ 543
Molecular weight
1104.15
Storage conditions
-20C
Shipping conditions
Ambient temperature

spectrum