AFDye™ 546 Azide (Alexa Fluor® 546 Azide equivalent) is a bright, orange-fluorescent dye that reacts with terminal alkynes via a copper-catalyzed click reaction (CuAAC) to form a stable triazole linker. It also reacts with strained cyclooctyne via a copper-free click chemistry reaction to form a stable triazole and does not require a Cu-catalyst or elevated temperatures.
AFDye™ 546 Azide is a water-soluble, pH-insensitive (from pH 4-10), orange-fluorescent dye with absorption and emission maxima at 554 and 570 nm, respectively. It can be used with the 488 nm and 532 nm laser lines. AFDye™ 546 conjugates to a variety of antibodies, peptides, proteins, tracers, and amplification substrates which are often used for generating stable signals in imaging and flow cytometry.
AFDye™ 546 dye is structurally identical to Alexa Fluor® 546 dye sold by Thermo Fisher Scientific.