AFDye 546 Azide

Catalog#: 1055-1
Unit size: 1 mg
In stock
Catalog#: 1055-5
Unit size: 5 mg
In stock
Catalog#: 1055-25
Unit size: 25 mg
In stock
Catalog#: 1055-100
Unit size: 100 mg
In stock

AFDye™ 546 Azide (Alexa Fluor® 546 Azide equivalent) is a bright, orange-fluorescent dye that reacts with terminal alkynes via a copper-catalyzed click reaction (CuAAC) to form a stable triazole linker. It also reacts with strained cyclooctyne via a copper-free click chemistry reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures.

Alexa Fluor 546 Azide is water-soluble, and pH-insensitive from pH 4 to pH 10 orange-fluorescent dye with absorption and emission maxima at 554 and 570 nm, respectively. It can be used with the 488 nm and 532 nm laser lines. AFDye™ 546 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates often used for generation of stable signal in imaging and flow cytometry.

AFDye™ 546 dye structurally is identical to Alexa Fluor® 546 dye sold by ThermoFisher Scintific.

Alkynes, cyclooctynes
Abs/Em Maxima
554/570 nm
Extinction coefficient
112,000 cm-1M-1
Water, DMSO, DMF
Spectrally similar dyes
Alexa Fluor® 546, TAMRA, CF™ 543, MB™ 543
Molecular weight
972.36 (protonated)
Storage conditions
Shipping conditions
Ambient temperature

Alexa Fluor® Dyes is a registered trademark of Invitrogen, part of Thermo Fisher Scientific. Fluoroprobes is not affiliated with or endorsed by Thermo Fisher Scientific. AFDyes are GENERIC versions of Alexa Fluor® Dyes and ARE NOT manufactures, distributed, or affiliated in any way with Thermo Fisher Scientific.