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AFDye 546 Azide

Catalog#
Unit Size
Price (USD)
Availability
Qty
Cat.#: 1055-1
Unit size: 1 mg
$139.00
in stock
Cat.#: 1055-5
Unit size: 5 mg
$445.00
in stock
Cat.#: 1055-25
Unit size: 25 mg
$1,395.00
in stock

AFDye™ 546 Azide (Alexa Fluor® 546 Azide equivalent) is a bright, orange-fluorescent dye that reacts with terminal alkynes via a copper-catalyzed click reaction (CuAAC) to form a stable triazole linker. It also reacts with strained cyclooctyne via a copper-free click chemistry reaction to form a stable triazole and does not require a Cu-catalyst or elevated temperatures.

AFDye™ 546 Azide is a water-soluble, pH-insensitive (from pH 4-10), orange-fluorescent dye with absorption and emission maxima at 554 and 570 nm, respectively. It can be used with the 488 nm and 532 nm laser lines. AFDye™ 546 conjugates to a variety of antibodies, peptides, proteins, tracers, and amplification substrates which are often used for generating stable signals in imaging and flow cytometry.

AFDye™ 546 dye is structurally identical to Alexa Fluor® 546 dye sold by Thermo Fisher Scientific.

Reactivity
Alkynes, cyclooctynes
Abs/Em Maxima
554/570 nm
Extinction coefficient
112,000 cm-1M-1
Solubility
Water, DMSO, DMF
Spectrally similar dyes
Alexa Fluor® 546, TAMRA, CF™ 543, MB™ 543
Molecular weight
972.36 (protonated)
Storage conditions
-20C
Shipping conditions
Ambient temperature

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