AZDye 532 NHS Ester

Catalog#

Unit Size

Price (USD)

Availability

Qty

Cat.#: 1041-1

Unit size: 1 mg

$149.00

in stock

Cat.#: 1041-5

Unit size: 5 mg

$465.00

in stock

Cat.#: 1041-25

Unit size: 25 mg

$1,495.00

in stock

Cat.#: 1041-100

Unit size: 100 mg

$2,795.00

in stock

AZDye™ 532 NHS Ester is an amine reactive, yellow-emitting dye routinely used to label proteins or antibodies through primary amines (Lys), amine-modified oligonucleotides, and other amine-containing biomolecules. The labeling occurs most efficiently at pH 7-9 and forms a stable, covalent amide bond.

AZDye™ 532 is a bright yellow-fluorescent dye with pH insensitive emission from pH 4 to pH 10. The excitation of AZDye™ 532 is ideally suited for the frequency-doubled Nd:YAG laser line. AZDye™ 532 can be conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates, and is often used for the generation of stable signals in imaging and flow cytometry.

AZDye™ 532 NHS Ester is structurally identical to Alexa Fluor® 532 NHS Ester sold by Thermo Fisher Scientific.

Specifications
Structure
Abs/Em Spectra
Protein Labeling Calculator
Documents

Reactivity

Primary amines

Abs/Em Maxima

530/555 nm

Extinction coefficient

81,000 cm-1M-1

Solubility

Water, DMSO, DMF

Spectrally similar dyes

Alexa Fluor® 532, DyLight® 532

Molecular weight

723.80

Storage conditions

-20C

Shipping conditions

Ambient temperature

Protein name

Protein molecular weight (Da)

Protein concentration (mg/mL)

Volume of protein to be labeled (µL)

Enter only one — either volume or amount of protein to be labeled

Amount of protein to be labeled (mg)

Molecular weight of labeling reagent (Da)

Amount of labeling reagent used (mg)

Maximum concentration of DMSO (%) *
* Leave blank or 0 if not used

Enter only one — either maximum concentration of DMSO or volume DMSO added to label

Volume of anhydrous DMSO added (µL)

Molar equivalents of labeling reagent used

Volume of DMSO required to make a stock solution (µL)

Volume of protein to be labeled (µL)

Amount of stock labeling reagent to be added (µL)

Amount of DMSO in labeling reaction (%)