AFDye 532 NHS Ester

Catalog#: 1041-1
Unit size: 1 mg
In stock
Catalog#: 1041-5
Unit size: 5 mg
In stock
Catalog#: 1041-25
Unit size: 25 mg
In stock
Catalog#: 1041-100
Unit size: 100 mg
In stock

AFDye™ 532 NHS Ester (Alexa Fluor® 532 NHS Ester equivalent) is an amine reactive, yellow emitting dye routinely used to label proteins or antibodies through the primary amines (Lys), amine-modified oligonucleotides, and other amine-containing biomolecules. The labeling occurs most efficiently at pH 7-9 and forms a stable, covalent amide bond.

AFDye™ 532 is a water-soluble, highly photostable, and bright yellow dye with pH insensitive emission from pH 4 to pH 10. The excitation of AFDye™ 532 ideally suited for the frequency-doubled Nd:YAG laser line. AFDye™ 532 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates often used for generation of stable signal in imaging and flow cytometry.

AFDye™ 532 NHS Ester structurally is identical to Alexa Fluor® 532 NHS Ester sold by ThermoFisher Scientific.

Primary amine
Abs/Em Maxima
530/555 nm
Extinction coefficient
81,000 cm-1M-1
Water, DMSO, DMF
Spectrally similar dyes
Alexa Fluor® 532, DyLight® 532
Molecular weight
Storage conditions
Shipping conditions
Ambient temperature

Enter only one — either volume or amount of protein to be labeled
Enter only one — either maximum concentration of DMSO or volume DMSO added to label

Alexa Fluor® Dyes is a registered trademark of Invitrogen, part of Thermo Fisher Scientific. Fluoroprobes is not affiliated with or endorsed by Thermo Fisher Scientific. AFDyes are GENERIC versions of Alexa Fluor® Dyes and ARE NOT manufactures, distributed, or affiliated in any way with Thermo Fisher Scientific.