AZDye 532 NHS Ester

Unit Size
Price (USD)
Cat.#: 1041-1
Unit size: 1 mg
in stock
Cat.#: 1041-5
Unit size: 5 mg
in stock
Cat.#: 1041-25
Unit size: 25 mg
in stock
Cat.#: 1041-100
Unit size: 100 mg
in stock

AZDye™ 532 NHS Ester is an amine reactive, yellow-emitting dye routinely used to label proteins or antibodies through primary amines (Lys), amine-modified oligonucleotides, and other amine-containing biomolecules. The labeling occurs most efficiently at pH 7-9 and forms a stable, covalent amide bond.

AZDye™ 532 is a bright yellow-fluorescent dye with pH insensitive emission from pH 4 to pH 10. The excitation of AZDye™ 532 is ideally suited for the frequency-doubled Nd:YAG laser line. AZDye™ 532 can be conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates, and is often used for the generation of stable signals in imaging and flow cytometry.

AZDye™ 532 NHS Ester is structurally identical to Alexa Fluor® 532 NHS Ester sold by Thermo Fisher Scientific.

Primary amines
Abs/Em Maxima
530/555 nm
Extinction coefficient
81,000 cm-1M-1
Water, DMSO, DMF
Spectrally similar dyes
Alexa Fluor® 532, DyLight® 532
Molecular weight
Storage conditions
Shipping conditions
Ambient temperature


Enter only one — either volume or amount of protein to be labeled
Enter only one — either maximum concentration of DMSO or volume DMSO added to label

Alexa Fluor® is a registered trademark of Thermo Fisher Scientific.