AFDye™ 532 NHS Ester (Alexa Fluor® 532 NHS Ester equivalent) is an amine reactive, yellow emitting dye routinely used to label proteins or antibodies through the primary amines (Lys), amine-modified oligonucleotides, and other amine-containing biomolecules. The labeling occurs most efficiently at pH 7-9 and forms a stable, covalent amide bond.
AFDye™ 532 is a water-soluble, highly photostable, and bright yellow dye with pH insensitive emission from pH 4 to pH 10. The excitation of AFDye™ 532 ideally suited for the frequency-doubled Nd:YAG laser line. AFDye™ 532 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates often used for generation of stable signal in imaging and flow cytometry.
AFDye™ 532 NHS Ester structurally is identical to Alexa Fluor® 532 NHS Ester sold by ThermoFisher Scientific.