AZDye 532 Maleimide

Unit Size
Price (USD)
Cat.#: 1042-1
Unit size: 1 mg
in stock
Cat.#: 1042-5
Unit size: 5 mg
in stock
Cat.#: 1042-25
Unit size: 25 mg
in stock
Cat.#: 1042-100
Unit size: 100 mg
in stock

AZDye™ 532 Maleimide is a water-soluble, photostable, bright yellow-fluorescent dye with pH insensitive emission from pH 4 to pH 10. The excitation of AZDye™ 532 is ideally suited for the frequency-doubled Nd:YAG laser line. AZDye™ 532 dye can be conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates, and is often used for the generation of stable signals in imaging and flow cytometry.

Maleimide is the most popular sulfhydryl-reactive group for conjugating the dye to a thiol group on a protein, oligonucleotide thiophosphate, or low molecular weight ligand. The maleimide group specifically and efficiently reacts with reduced thiols (sulfhydryl groups, –SH) at pH 6.5 to 7.5 to form a stable thioether bond. The resulting conjugates exhibit brighter fluorescence and greater photostability than the conjugates of many other spectrally similar fluorophores.

AZDye™ 532 Maleimide dye is structurally identical to Alexa Fluor™ 532 C5 Maleimide.

Thiol (Cys)
Abs/Em Maxima
530/555 nm
Extinction coefficient
78,000 cm-1M-1
Water, DMSO, DMF
Spectrally similar dyes
Alexa Fluor® 532, DyLight® 532
Molecular weight
790.90 (protonated)
Storage conditions
Shipping conditions
Ambient temperature


Enter only one — either volume or amount of protein to be labeled
Enter only one — either maximum concentration of DMSO or volume DMSO added to label

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