AFDye 532 Maleimide

Catalog#: 1042-1
Unit size: 1 mg
$149.00
In stock
Catalog#: 1042-5
Unit size: 5 mg
$395.00
In stock
Catalog#: 1042-25
Unit size: 25 mg
$1,495.00
In stock
Catalog#: 1042-100
Unit size: 100 mg
$2,795.00
In stock

AFDye™ 532 Maleimide (Alexa Fluor® 532 C5 Maleimide equivalent) is a water-soluble, photostable, bright yellow-fluorescent dye with pH insensitive emission from pH 4 to pH 10. The excitation of AFDye™ 532 is ideally suited for the frequency-doubled Nd:YAG laser line. AFDye™ 532 dye can be conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates, and is often used for the generation of stable signals in imaging and flow cytometry.

Maleimide is the most popular sulfhydryl-reactive group for conjugating the dye to a thiol group on a protein, oligonucleotide thiophosphate, or low molecular weight ligand. The maleimide group specifically and efficiently reacts with reduced thiols (sulfhydryl groups, –SH) at pH 6.5 to 7.5 to form a stable thioether bond. The resulting conjugates exhibit brighter fluorescence and greater photostability than the conjugates of many other spectrally similar fluorophores.

AFDye™ 532 Maleimide dye is structurally identical to Alexa Fluor™ 532 C5 Maleimide sold by Thermo Fisher Scientific.

Reactivity
Thiol (Cys)
Abs/Em Maxima
530/555 nm
Extinction coefficient
78,000 cm-1M-1
Solubility
Water, DMSO, DMF
Spectrally similar dyes
Alexa Fluor® 532, DyLight® 532
Molecular weight
812.88
Storage conditions
-20C
Shipping conditions
Ambient temperature

Enter only one — either volume or amount of protein to be labeled
Enter only one — either maximum concentration of DMSO or volume DMSO added to label

Alexa Fluor® is a registered trademark of Invitrogen, part of Thermo Fisher Scientific. Fluoroprobes is not affiliated with or endorsed by Thermo Fisher Scientific. AFDyes™ are GENERIC versions of Alexa Fluor® Dyes and ARE NOT manufactured, distributed, or affiliated in any way with Thermo Fisher Scientific.