AFDye 532 Maleimdie

Catalog#: 1042-1
Unit size: 1 mg
In stock
Catalog#: 1042-5
Unit size: 5 mg
In stock
Catalog#: 1042-25
Unit size: 25 mg
In stock
Catalog#: 1042-100
Unit size: 100 mg
In stock

AFDye™ 532 Maleimide (Alexa Fluor® 532 C5 Maleimide equivalent) is a water-soluble, photostable, and bright yellow-fluorescent dye with pH insensitive emission from pH 4 to pH 10. The excitation of AFDye™ 532 ideally suited for the frequency-doubled Nd:YAG laser line. AFDye™ 532 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates often used for generation of stable signal in imaging and flow cytometry.

Maleimide is the most popular sulfhydryl-reactive reactive group for conjugating the dye to a thiol group on a protein, oligonucleotide thiophosphate, or low molecular weight ligand. The maleimide group specifically and efficiently reacts with reduced thiols (sulfhydryl groups, –SH) at pH 6.5 to 7.5 to form stable thioether bond. The resulting conjugates exhibit brighter fluorescence and greater photostability than the conjugates of many other spectrally similar fluorophores.

AFDye™ 532 Maleimdie dye structurally is identical Alexa Fluor™ 532 C5 Maleimide sold by ThermoFisher Scientific.

Thiol (Cys)
Abs/Em Maxima
530/555 nm
Extinction coefficient
78,000 cm-1M-1
Water, DMSO, DMF
Spectrally similar dyes
Alexa Fluor® 532, DyLight® 532
Molecular weight
Storage conditions
Shipping conditions
Ambient temperature

Enter only one — either volume or amount of protein to be labeled
Enter only one — either maximum concentration of DMSO or volume DMSO added to label

Alexa Fluor® Dyes is a registered trademark of Invitrogen, part of Thermo Fisher Scientific. Fluoroprobes is not affiliated with or endorsed by Thermo Fisher Scientific. AFDyes are GENERIC versions of Alexa Fluor® Dyes and ARE NOT manufactures, distributed, or affiliated in any way with Thermo Fisher Scientific.