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AFDye 532 DBCO

Catalog#
Unit Size
Price (USD)
Availability
Qty
Cat.#: 1046-1
Unit size: 1 mg
$149.00
in stock
Cat.#: 1046-5
Unit size: 5 mg
$475.00
in stock
Cat.#: 1046-25
Unit size: 25 mg
$1,495.00
in stock

AFDye™ 532 DBCO (Alexa Fluor® 532 DIBO analog) is an azide-reactive probe that can be used for imaging azide-tagged biomolecules via a copper-free click reaction. DBCO moiety reacts with azides to form a stable triazole and does not require a Cu-catalyst or elevated temperatures. In applications where the presence of copper is a concern, AFDye™ 532 DBCO is an ideal alternative to copper-requiring fluorescent alkynes.

AFDye™ 532 DBCO is ideally suited for the frequency-doubled Nd:YAG laser line. This probe is water-soluble and its fluorescence is pH independent over a wide pH range. The brightness and photostability of AFDyes™ 532 are well suited to direct imaging of low-abundance targets.

Reactivity
Azides
Abs/Em Maxima
530/555 nm
Extinction coefficient
81,000 cm-1M-1
Solubility
Water, DMSO, DMF
Spectrally similar dyes
Alexa Fluor® 532, Atto™ 532, CF® 532, DyLight® 532
Molecular weight
ca. 825
Storage conditions
-20C
Shipping conditions
Ambient temperature

Available upon request

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