AFDye 532 DBCO

Catalog#: 1046-1
Unit size: 1 mg
In stock
Catalog#: 1046-5
Unit size: 5 mg
In stock
Catalog#: 1046-25
Unit size: 25 mg
In stock
Catalog#: 1046-100
Unit size: 100 mg
In stock

AFDye™ 532 DBCO (Alexa Fluor® 532 DIBO analog) an azide reactive probe that can be used for imaging azide-tagged biomolecules via a copper-free click reaction. DBCO moiety reacts with azides to form a stable triazole and does not require Cu-catalyst or elevated temperatures. In application where the presence of copper is a concern AFDye™ 532 DBCO is an ideal alternative to copper requiring fluorescent alkynes.

AFDye™ 532 DBCO is ideally suited for the frequency-doubled Nd:YAG laser line. This probe is water-soluble and its fluorescence is pH independent over a wide pH range. The brightness and photostability of AF 532 dyes are well suited to direct imaging of low-abundance targets.

Abs/Em Maxima
530/555 nm
Extinction coefficient
81,000 cm-1M-1
Water, DMSO, DMF
Spectrally similar dyes
Alexa Fluor® 532, Atto™ 532, CF® 532, DyLight® 532
Molecular weight
ca. 825
Storage conditions
Shipping conditions
Ambient temperature

Available upon request

Alexa Fluor® Dyes is a registered trademark of Invitrogen, part of Thermo Fisher Scientific. Fluoroprobes is not affiliated with or endorsed by Thermo Fisher Scientific. AFDyes are GENERIC versions of Alexa Fluor® Dyes and ARE NOT manufactures, distributed, or affiliated in any way with Thermo Fisher Scientific.