AFDye™ 532 Azide (Alexa Fluor® 532 Azide analog) is a bright, yellow-fluorescent dye that reacts with terminal alkynes via a copper-catalyzed click reaction (CuAAC) to form a stable triazole linker. It also reacts with strained cyclooctyne via a copper-free click chemistry reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures.
AFDye™ 532 Azide is ideally suited for the frequency-doubled Nd:YAG laser line. This probe is water-soluble and its fluorescence is pH independent over a wide pH range. The brightness and photostability of AFDye 532 probes are well suited to direct imaging of low-abundance targets.