AFDye 532 Azide

Unit Size
Price (USD)
Cat.#: 1043-1
Unit size: 1 mg
in stock
Cat.#: 1043-5
Unit size: 5 mg
in stock
Cat.#: 1043-25
Unit size: 25 mg
in stock

AFDye™ 532 Azide (Alexa Fluor® 532 Azide analog) is a bright, yellow-fluorescent dye that reacts with terminal alkynes via a copper-catalyzed click reaction (CuAAC) to form a stable triazole linker. It also reacts with strained cyclooctyne via a copper-free click chemistry reaction to form a stable triazole and does not require a Cu-catalyst or elevated temperatures.

AFDye™ 532 Azide is ideally suited for the frequency-doubled Nd:YAG laser line. This probe is water-soluble and its fluorescence is pH independent over a wide pH range. The brightness and photostability of AFDye 532 probes are well suited to direct imaging of low-abundance targets.

AFDye™ 532 is spectrally identical to Alexa Fluor® 532 and CF®532 Dye.

Alkynes, cyclooctynes
Abs/Em Maxima
530/555 nm
Extinction coefficient
81,000 cm-1M-1
Water, DMSO, DMF
Spectrally similar dyes
Alexa Fluor® 532, DyLight® 532
Molecular weight
Storage conditions
Shipping conditions
Ambient temperature

Available upon request

Alexa Fluor® is a registered trademark of Invitrogen, part of Thermo Fisher Scientific. Fluoroprobes is not affiliated with or endorsed by Thermo Fisher Scientific. AFDyes™ are GENERIC versions of Alexa Fluor® Dyes and ARE NOT manufactured, distributed, or affiliated in any way with Thermo Fisher Scientific.