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AFDye 532 Alkyne
Catalog#:
1045-1
Unit size:
1 mg
$129.00
In stock
Catalog#:
1045-5
Unit size:
5 mg
$395.00
In stock
Catalog#:
1045-25
Unit size:
25 mg
$1,095.00
In stock
Catalog#:
1045-100
Unit size:
100 mg
$2,395.00
In stock
AFDye™ 532 Alkyne (Alexa Fluor® 532 Alkyne equivalent) is a bright, yellow-fluorescent alkyne-activated probe routinely used for imaging of azide-containing biomolecules. AFDye™ 532 Alkyne reacts with azides via a copper-catalyzed click reaction (CuAAC) to form a stable triazole linker. A probe for copper-less detection, AF™ 532 DBCO is also available for application where the presence of copper is not acceptable.
AFDye™ 532 Alkyne is ideally suited for the frequency-doubled Nd:YAG laser line. This probe is water-soluble and its fluorescence is pH independent over a wide pH range. The brightness and photostability of AF 532 dyes are well suited to direct imaging of low-abundance targets.
AFDye™ 532 spectrally is identical to Alexa Fluor® 532 sold by ThermoFisher Scientific and CF®532 Dye.
Reactivity
Azide
Abs/Em Maxima
530/555 nm
Extinction coefficient
81,000 cm-1M-1
Solubility
Water, DMSO, DMF
Spectrally similar dyes
Alexa Fluor® 532, DyLight® 532
Molecular weight
855.35
Storage conditions
-20C
Shipping conditions
Ambient temperature
Available upon request
Alexa Fluor® Dyes is a registered trademark of Invitrogen, part of Thermo Fisher Scientific. Fluoroprobes is not affiliated with or endorsed by Thermo Fisher Scientific. AFDyes are GENERIC versions of Alexa Fluor® Dyes and ARE NOT manufactures, distributed, or affiliated in any way with Thermo Fisher Scientific.
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