AFDye 532 Alkyne

Catalog#: 1045-1
Unit size: 1 mg
$129.00
In stock
Catalog#: 1045-5
Unit size: 5 mg
$395.00
In stock
Catalog#: 1045-25
Unit size: 25 mg
$1,095.00
In stock
Catalog#: 1045-100
Unit size: 100 mg
$2,395.00
In stock

AFDye™ 532 Alkyne (Alexa Fluor® 532 Alkyne equivalent) is a bright, yellow-fluorescent alkyne-activated probe routinely used for imaging of azide-containing biomolecules. AFDye™ 532 Alkyne reacts with azides via a copper-catalyzed click reaction (CuAAC) to form a stable triazole linker. A probe for copper-less detection, AF™ 532 DBCO is also available for application where the presence of copper is not acceptable.

AFDye™ 532 Alkyne is ideally suited for the frequency-doubled Nd:YAG laser line. This probe is water-soluble and its fluorescence is pH independent over a wide pH range. The brightness and photostability of AF 532 dyes are well suited to direct imaging of low-abundance targets.

AFDye™ 532 spectrally is identical to Alexa Fluor® 532 sold by ThermoFisher Scientific and CF®532 Dye.

Reactivity
Azide
Abs/Em Maxima
530/555 nm
Extinction coefficient
81,000 cm-1M-1
Solubility
Water, DMSO, DMF
Spectrally similar dyes
Alexa Fluor® 532, DyLight® 532
Molecular weight
855.35
Storage conditions
-20C
Shipping conditions
Ambient temperature

Available upon request

Alexa Fluor® Dyes is a registered trademark of Invitrogen, part of Thermo Fisher Scientific. Fluoroprobes is not affiliated with or endorsed by Thermo Fisher Scientific. AFDyes are GENERIC versions of Alexa Fluor® Dyes and ARE NOT manufactures, distributed, or affiliated in any way with Thermo Fisher Scientific.