AFDye™ 532 Alkyne (Alexa Fluor® 532 Alkyne equivalent) is a bright, yellow-fluorescent alkyne-activated probe routinely used for imaging of azide-containing biomolecules. AFDye™ 532 Alkyne reacts with azides via a copper-catalyzed click reaction (CuAAC) to form a stable triazole linker. A probe for copper-less detection, AF™ 532 DBCO is also available for application where the presence of copper is not acceptable.
AFDye™ 532 Alkyne is ideally suited for the frequency-doubled Nd:YAG laser line. This probe is water-soluble and its fluorescence is pH independent over a wide pH range. The brightness and photostability of AF 532 dyes are well suited to direct imaging of low-abundance targets.