• SIGN IN
  • No products in the cart.

AFDye 532 Alkyne

Catalog#
Unit Size
Price (USD)
Availability
Qty
Cat.#: 1045-1
Unit size: 1 mg
$139.00
in stock
Cat.#: 1045-5
Unit size: 5 mg
$445.00
in stock
Cat.#: 1045-25
Unit size: 25 mg
$1,395.00
in stock

AFDye™ 532 Alkyne (Alexa Fluor® 532 Alkyne equivalent) is a bright, yellow-fluorescent, alkyne-activated probe routinely used for imaging of azide-containing biomolecules. AFDye™ 532 Alkyne reacts with azides via a copper-catalyzed click reaction (CuAAC) to form a stable triazole linker. A probe for copper-less detection, AFDye™ 532 DBCO, is also available for applications where the presence of copper is undesirable.

AFDye™ 532 Alkyne is ideally suited for the frequency-doubled Nd:YAG laser line. This probe is water-soluble and its fluorescence is pH independent over a wide pH range. The brightness and photostability of AF 532 dyes are well suited to direct imaging of low-abundance targets.

AFDye™ 532 is spectrally identical to Alexa Fluor® 532, as well as CF®532 Dye.

Reactivity
Azides
Abs/Em Maxima
530/555 nm
Extinction coefficient
81,000 cm-1M-1
Solubility
Water, DMSO, DMF
Spectrally similar dyes
Alexa Fluor® 532, DyLight® 532
Molecular weight
855.35
Storage conditions
-20C
Shipping conditions
Ambient temperature

Available upon request

spectrum