AZDye 488 Picolyl Azide

Unit Size
Price (USD)
Cat.#: 1019-1
Unit size: 1 mg
in stock
Cat.#: 1019-5
Unit size: 5 mg
in stock
Cat.#: 1019-25
Unit size: 25 mg
in stock

AZDye™ 488 Picolyl Azide is an advanced fluorescent probe that incorporates a copper-chelating motif to raise the effective concentration of Cu(I) at the reaction site. The rate of the CuAAC reaction depends on the concentrations of all reagents, including copper, therefore raising the effective copper concentration at the reaction site can dramatically increase the rate of reaction without the need to increase the concentration of azide reagent or copper.

In addition, the use of picolyl azides instead of conventional azides allows for at least a tenfold reduction in the concentration of the copper catalyst without sacrificing the efficiency of labeling.

In summary, the introduction of a picolyl moiety into an azide probe leads to a substantial increase in the sensitivity of alkyne detection. This is of special value for the detection of low abundance targets or where significant increase in signal intensity is desired.

Alkynes, cyclooctynes
Abs/Em Maxima
494/517 nm
Extinction coefficient
73,000 cm-1M-1
Water, DMSO, DMF
Spectrally similar dyes
Alexa Fluor® 488, DyLight® 488, Fluorescein, Oregon Green 488
Molecular weight
736.69 (protonated)
Storage conditions
Shipping conditions
Ambient temperature

Alexa Fluor® is a registered trademark of Thermo Fisher Scientific.