AFDye 488 NHS ester

Catalog#: 1013-1
Unit size: 1 mg
$129.00
In stock
Catalog#: 1013-5
Unit size: 5 mg
$395.00
In stock
Catalog#: 1013-25
Unit size: 25 mg
$1,295.00
In stock
Catalog#: 1013-100
Unit size: 100 mg
$2,495.00
In stock

AFDye™ 488 NHS Ester (Alexa Fluor 488 NHS Ester equivalent) is an amine reactive, green emitting dye routinely used to label proteins or antibodies through the primary amines (Lys), amine-modified oligonucleotides, and other amine-containing biomolecules. The labeling occurs most efficiently at pH 7-9 and forms a stable, covalent amide bond.

AFDye™ 488 is a water-soluble, highly photostable dye with very high fluorescence quantum yield. Its green fluorescence is pH independent from pH 4 to pH 10. The excitation of AFDye™ ideally suited for the 488 nm line. AFDye™ 488 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates often used for generation of stable signal in imaging and flow cytometry.

AFDye™ 488 dye structurally is identical to Invitrogen’s Alexa Fluor® 488

Reactivity
Primary amine
Abs/Em Maxima
494/517 nm
Extinction coefficient
73,000 cm-1M-1
Solubility
Water, DMSO, DMF
Spectrally similar dyes
Alexa Fluor® 488, DyLight® 488, Fluorescein, Oregon Green 488
Molecular weight
833.26
Storage conditions
-20C
Shipping conditions
Ambient temperature

Enter only one — either volume or amount of protein to be labeled
Enter only one — either maximum concentration of DMSO or volume DMSO added to label

Alexa Fluor® Dyes is a registered trademark of Invitrogen, part of Thermo Fisher Scientific. Fluoroprobes is not affiliated with or endorsed by Thermo Fisher Scientific. AFDyes are GENERIC versions of Alexa Fluor® Dyes and ARE NOT manufactures, distributed, or affiliated in any way with Thermo Fisher Scientific.