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AFDye 488 NHS ester

Catalog#
Unit Size
Price (USD)
Availability
Qty
Cat.#: 1013-1
Unit size: 1 mg
$139.00
in stock
Cat.#: 1013-5
Unit size: 5 mg
$395.00
in stock
Cat.#: 1013-25
Unit size: 25 mg
$1,395.00
in stock
Cat.#: 1013-100
Unit size: 100 mg
$2,495.00
in stock

AFDye™ 488 NHS Ester (Alexa Fluor® 488 NHS Ester equivalent) is an amine reactive, green-emitting dye routinely used to label proteins or antibodies through primary amines (Lys), amine-modified oligonucleotides, and other amine-containing biomolecules. The labeling occurs most efficiently at pH 7-9 and forms a stable, covalent amide bond.

AFDye™ 488 is a water-soluble, highly photostable dye with a very high fluorescence quantum yield. AFDye 488 has green fluorescence that is pH independent from pH 4 to pH 10 with excitation ideally suited for the 488 nm laser line. AFDye™ 488 dye can be conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates which are often used for generation of stable signals in imaging and flow cytometry.

Reactivity
Primary amines
Abs/Em Maxima
494/517 nm
Extinction coefficient
73,000 cm-1M-1
Solubility
Water, DMSO, DMF
Spectrally similar dyes
Alexa Fluor® 488, DyLight® 488, Fluorescein, Oregon Green 488
Molecular weight
631.54 (protonated)
Storage conditions
-20C
Shipping conditions
Ambient temperature

spectrum

Enter only one — either volume or amount of protein to be labeled
Enter only one — either maximum concentration of DMSO or volume DMSO added to label

Alexa Fluor® is a registered trademark of Thermo Fisher Scientific.