AZDye 488 NHS Ester

Unit Size
Price (USD)
Cat.#: 1013-1
Unit size: 1 mg
in stock
Cat.#: 1013-5
Unit size: 5 mg
in stock
Cat.#: 1013-25
Unit size: 25 mg
in stock
Cat.#: 1013-100
Unit size: 100 mg
in stock

AZDye™ 488 NHS Ester is an amine reactive, green-emitting dye routinely used to label proteins or antibodies through primary amines (Lys), amine-modified oligonucleotides, and other amine-containing biomolecules. The labeling occurs most efficiently at pH 7-9 and forms a stable, covalent amide bond.

AZDye™ 488 is a water-soluble, highly photostable dye with a very high fluorescence quantum yield. AZDye 488 has green fluorescence that is pH independent from pH 4 to pH 10 with excitation ideally suited for the 488 nm laser line. AZDye™ 488 dye can be conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates which are often used for generation of stable signals in imaging and flow cytometry.

AZDye™ 488 Ester is structurally identical to Alexa Fluor® 488 NHS Ester. Its absorption/emission spectra is a perfect match to spectra of many other fluorescent dyes based on sulfonated rhodamine 110 core, including DyLight® 488, Alexa Fluor® 488, and CF® 488 Dye.

DyLight® 488 and Alexa Fluor® is a registered trademark of Thermo Fisher Scientific.

Primary amines
Abs/Em Maxima
494/517 nm
Extinction coefficient
73,000 cm-1M-1
Water, DMSO, DMF
Spectrally similar dyes
Alexa Fluor® 488, DyLight® 488, Fluorescein, Oregon Green 488
Molecular weight
631.54 (protonated)
Storage conditions
Shipping conditions
Ambient temperature


Enter only one — either volume or amount of protein to be labeled
Enter only one — either maximum concentration of DMSO or volume DMSO added to label

Alexa Fluor® is a registered trademark of Thermo Fisher Scientific.