AFDye 488 DBCO

Catalog#: 1021-1
Unit size: 1 mg
$139.00
In stock
Catalog#: 1021-5
Unit size: 5 mg
$395.00
In stock
Catalog#: 1021-25
Unit size: 25 mg
$1,295.00
In stock
Catalog#: 1021-100
Unit size: 100 mg
$2,895.00
In stock

AFDye™ 488 DBCO (Alexa Fluor® 488 DIBO analog) reacts with azides via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. In application where the presence of copper is a concern Alexa Fluor 488 DBCO is an ideal alternative to copper requiring fluorescent alkynes.

AFDye™ 488 is a bright, and highly photostable, green-fluorescent probe optimally excited by the 488 nm laser line. This probe is water-soluble and its fluorescence is pH independent over a wide pH range. The brightness and photostability of blue dyes are best suited to direct imaging of low-abundance targets.

AFDye™ 488 dye structurally is identical to Alexa Fluor® 488 sold by ThermoFisher Scientific.

Reactivity
Azides
Abs/Em Maxima
494/517 nm
Extinction coefficient
73,000 cm-1M-1
Solubility
Water, DMSO, DMF
Spectrally similar dyes
Alexa Fluor® 488, DyLight® 488, Fluorescein, Oregon Green 488
Molecular weight
995.18
Storage conditions
-20C
Shipping conditions
Ambient temperature

Alexa Fluor® Dyes is a registered trademark of Invitrogen, part of Thermo Fisher Scientific. Fluoroprobes is not affiliated with or endorsed by Thermo Fisher Scientific. AFDyes are GENERIC versions of Alexa Fluor® Dyes and ARE NOT manufactures, distributed, or affiliated in any way with Thermo Fisher Scientific.