AFDye™ 488 Cadaverine (Alexa Fluor® 488 Cadaverine equivalent) is a carbonyl reactive building block used to modify carboxylic groups in the presence of activators (e.g. EDC, or DCC) or activated esters (e.g. NHS esters) through a stable amide bond. It also can be employed as a polar tracer and as a reactive dye for labeling proteins via a carboxylic acid moiety.
AFDye™ 488 is a water-soluble, bright, green-fluorescent dye with excitation ideally suited to the 488 nm laser line. Its green fluorescence is pH independent from pH 4 to pH 10. AF™ 488 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates often used for generation of stable signal in imaging and flow cytometry.
AFDye™ 488 dye structurally is identical to Alexa Fluor® 488 Cadaverine sold by ThermoFisher Scientific.