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AFDye 488 Azide

Catalog#
Unit Size
Price (USD)
Availability
Qty
Cat.#: 1018-1
Unit size: 1 mg
$139.00
in stock
Cat.#: 1018-5
Unit size: 5 mg
$445.00
in stock
Cat.#: 1018-25
Unit size: 25 mg
$1,395.00
in stock

AFDye™ 488 Azide (Alexa Fluor® 488 Azide equivalent) is a bright, green-fluorescent, azide-activated probe that reacts with terminal alkynes via a copper-catalyzed click reaction (CuAAC). It also reacts with strained cyclooctynes via a copper-free click chemistry reaction to form a stable triazole and does not require a Cu-catalyst or elevated temperatures.

AFDye™ 488 (Alexa Fluor® 488 equivalent) is a bright, highly photostable, green-fluorescent probe optimally excited by the 488 nm laser line. This probe is water-soluble and its fluorescence is pH independent over a wide pH range. The brightness and photostability of AFDye™ 488 dyes are best suited to direct imaging of low-abundance targets.

Reactivity
Alkynes, cyclooctynes
Abs/Em Maxima
494/517 nm
Extinction coefficient
73,000 cm-1M-1
Solubility
Water, DMSO, DMF
Spectrally similar dyes
Alexa Fluor® 488, DyLight® 488, Fluorescein, Oregon Green 488
Molecular weight
861.04
Storage conditions
-20C
Shipping conditions
Ambient temperature

spectrum
Alexa Fluor® is a registered trademark of Invitrogen, part of Thermo Fisher Scientific. Fluoroprobes is not affiliated with or endorsed by Thermo Fisher Scientific. AFDyes™ are GENERIC versions of Alexa Fluor® Dyes and ARE NOT manufactured, distributed, or affiliated in any way with Thermo Fisher Scientific.