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AFDye 488 Alkyne

Catalog#
Unit Size
Price (USD)
Availability
Qty
Cat.#: 1020-1
Unit size: 1 mg
$145.00
in stock
Cat.#: 1020-5
Unit size: 5 mg
$445.00
in stock
Cat.#: 1020-25
Unit size: 25 mg
$1,395.00
in stock

AFDye™ 488 Alkyne (Alexa Fluor® 488 Alkyne equivalent) is a bright, green-fluorescent, alkyne-activated probe routinely used for imaging of low abundance azide-containing biomolecules. AFDye™ 488 Alkyne reacts with azides via a copper-catalyzed click reaction (CuAAC) to form a stable triazole linker.

AFDye™ 488 is a bright, highly photostable, green-fluorescent probe optimally excited by the 488 nm laser line. This probe is water-soluble and its fluorescence is pH independent over a wide pH range. The brightness and photostability of AFDye™ 488 is best suited to direct imaging of low-abundance targets.

AFDye™ 488 Alkyne is structurally identical to Alexa Fluor® 488 Alkyne.

Reactivity
Azides
Abs/Em Maxima
494/517 nm
Extinction coefficient
73,000 cm-1M-1
Solubility
Water, DMSO, DMF
Spectrally similar dyes
Alexa Fluor® 488, DyLight® 488, Fluorescein, Oregon Green 488
Molecular weight
773.91
Storage conditions
-20C
Shipping conditions
Ambient temperature

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