AFDye™ 430 DBCO (Alexa Fluor® 430 DIBO) reacts with azides via a copper-free click chemistry reaction to form a stable triazole and does not require a Cu-catalyst or elevated temperatures. In applications where the presence of copper is a concern, AFDye™ 430 DBCO is an ideal alternative to copper requiring fluorescent alkynes.
AFDye™ 430 is a bright, photostable, green-fluorescent probe optimally excited near its absorption maximum at 432 nm. Its emission peak at 539 nm is pH independent over a wide pH range.
AFDye™ DBCO reagent is not suitable for staining intracellular components of fixed and permeabilized cells due to high backgrounds.
AFDye™ 430 dye is structurally identical to Invitrogen’s Alexa Fluor® 430 dye.