AFDye™ 430 Alkyne (Alexa Fluor 430 Alkyne) is a green-fluorescent alkyne-activated probe routinely used for imaging of moderate to high abundance azide-containing biomolecules. AFDye™ 430 Alkyne reacts with azides via a copper-catalyzed click reaction (CuAAC) to form a stable triazole linker.
AFDye™ 430 is a bright, and photostable, green-fluorescent probe optimally excited at near 430 nm. This probe is water-soluble and its fluorescence is pH independent over a wide pH range. The brightness and photostability of blue dyes are well suited to direct imaging of moderate to high abundance targets.
AFDye™ 430 dye structurally is identical to Invitrogen’s Alexa Fluor® 430