AFDye 405 Picolyl Azide

Catalog#: 1065-1
Unit size: 1 mg
$149.00
In stock
Catalog#: 1065-5
Unit size: 5 mg
$445.00
In stock
Catalog#: 1065-25
Unit size: 25 mg
$1,495.00
In stock

AFDye 405 Picolyl Azide is an advanced fluorescent probe that incorporates a copper-chelating motif to raise the effective concentration of Cu(I) at the reaction site. The rate of the CuAAC reaction depends on the concentrations of all reagents, including copper, therefore raising the effective copper concentration at the reaction site dramatically increases the rate of CuAAC reaction without increasing the concentrations of azide reagent or copper.

In addition, the use of picolyl azide instead of conventional azides allows for at least a tenfold reduction in the concentration of the copper catalyst without sacrificing the efficiency of labeling.

In summary, the introduction of a picolyl moiety into an azide probe leads to a substantial increase in the sensitivity of alkyne detection. This is of special value for the detection of low abundance targets or where significant increase in signal intensity is desired.

Reactivity
Alkynes, cyclooctynes
Abs/Em Maxima
402/424 nm
Extinction coefficient
35,000 cm-1M-1
Solubility
Water, DMSO, DMF
Spectrally similar dyes
Alexa Fluor® 405, DyLight® 405,
Molecular weight
718.68 (protonated) 1022.26 (triethylammonium salt)
Storage conditions
-20C
Shipping conditions
Ambient temperature

 

Alexa Fluor® is a registered trademark of Invitrogen, part of Thermo Fisher Scientific. Fluoroprobes is not affiliated with or endorsed by Thermo Fisher Scientific. AFDyes™ are GENERIC versions of Alexa Fluor® Dyes and ARE NOT manufactured, distributed, or affiliated in any way with Thermo Fisher Scientific.