AFDye 405 NHS Ester

Unit Size
Price (USD)
Cat.#: 1061-1
Unit size: 1 mg
in stock
Cat.#: 1061-5
Unit size: 5 mg
in stock
Cat.#: 1061-25
Unit size: 25 mg
in stock
Cat.#: 1061-100
Unit size: 100 mg
in stock

propertiesAFDye™ 405 NHS Ester (Alexa Fluor® 405 NHS ester) is a water-soluble, blue-fluorescent dye that is often used in multi-color applications, including flow cytometry and super-resolution microscopy using STORM. Its excitation is ideally suited for the 407 nm spectral line of the krypton laser or the 408 nm violet laser diode. AFDye™ 405 conjugates are pH-insensitive from pH 4 to pH 10.

The NHS ester (or succinimidyl ester) is the most popular amine reactive group for labeling with the primary amines of proteins (Lys), amine-modified oligonucleotides, and other amine-containing molecules. Proteins can be labeled with AFDye™ 405 NHS Ester at high molar ratios without significant self-quenching, leading to brighter conjugates and more sensitive detection. AFDye™ 405 conjugates can also be used for the detection of abundance targets.

AFDye™ 405 dye is structurally identical to Invitrogen’s Alexa Fluor® 405 Dye.

Primary amines
Abs/Em Maxima
402/424 nm
Extinction coefficient
33,000 cm-1M-1
Water, DMSO, DMF
Spectrally similar dyes
Alexa Fluor® 405, DyLight® 405, Cascade Blue
Molecular weight
Storage conditions
Shipping conditions
Ambient temperature



Enter only one — either volume or amount of protein to be labeled
Enter only one — either maximum concentration of DMSO or volume DMSO added to label

Alexa Fluor® is a registered trademark of Invitrogen, part of Thermo Fisher Scientific. Fluoroprobes is not affiliated with or endorsed by Thermo Fisher Scientific. AFDyes™ are GENERIC versions of Alexa Fluor® Dyes and ARE NOT manufactured, distributed, or affiliated in any way with Thermo Fisher Scientific.