AZDye 405 NHS Ester

Catalog#

Unit Size

Price (USD)

Availability

Qty

Cat.#: 1061-1

Unit size: 1 mg

$139.00

in stock

Cat.#: 1061-5

Unit size: 5 mg

$430.00

in stock

Cat.#: 1061-25

Unit size: 25 mg

$1,195.00

in stock

Cat.#: 1061-100

Unit size: 100 mg

$2,290.00

in stock

AZDye™ 405 NHS Ester is a water-soluble, blue-fluorescent dye that is often used in multi-color applications, including flow cytometry and super-resolution microscopy using STORM. Its excitation is ideally suited for the 407 nm spectral line of the krypton laser or the 408 nm violet laser diode. AZDye™ 405 conjugates are pH-insensitive from pH 4 to pH 10.

The NHS ester (or succinimidyl ester) is the most popular amine reactive group for labeling with the primary amines of proteins (Lys), amine-modified oligonucleotides, and other amine-containing molecules. Proteins can be labeled with AZDye™ 405 NHS Ester at high molar ratios without significant self-quenching, leading to brighter conjugates and more sensitive detection.

Specifications
Structure
Abs/Em Spectra
Protein Labeling Calculator
Documents

Reactivity

Primary amines

Abs/Em Maxima

402/424 nm

Extinction coefficient

33,000 cm-1M-1

Solubility

Water, DMSO, DMF

Spectrally similar dyes

Alexa Fluor® 405, DyLight® 405, Cascade Blue

Molecular weight

726.05 (protonated)

Storage conditions

-20C

Shipping conditions

Ambient temperature

Protein name

Protein molecular weight (Da)

Protein concentration (mg/mL)

Volume of protein to be labeled (µL)

Enter only one — either volume or amount of protein to be labeled

Amount of protein to be labeled (mg)

Molecular weight of labeling reagent (Da)

Amount of labeling reagent used (mg)

Maximum concentration of DMSO (%) *
* Leave blank or 0 if not used

Enter only one — either maximum concentration of DMSO or volume DMSO added to label

Volume of anhydrous DMSO added (µL)

Molar equivalents of labeling reagent used

Volume of DMSO required to make a stock solution (µL)

Volume of protein to be labeled (µL)

Amount of stock labeling reagent to be added (µL)

Amount of DMSO in labeling reaction (%)