AFDye™ 405 DBCO (Alexa Fluor 405 DIBO analog) reacts with azides via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. In application where the presence of copper is a concern AFDye™ 405 DBCO is an ideal alternative to copper requiring fluorescent alkynes.
AFDye™ 405 (Alexa Fluor® 405) is a bright, blue-fluorescent probe optimally excited by the 407 nm spectral line of the krypton laser or the 408 nm violet laser diode. This probe is water-soluble and its fluorescence is pH independent over a wide pH range. The brightness and photostability of this dye are best suited to direct imaging of moderate-abundance targets.
AFDye™ 405 dye is structurally identical to Invitrogen’s Alexa Fluor® 405