AFDye 405 DBCO

Unit Size
Price (USD)
Cat.#: 1066-1
Unit size: 1 mg
in stock
Cat.#: 1066-5
Unit size: 5 mg
in stock
Cat.#: 1066-25
Unit size: 25 mg
in stock

AFDye™ 405 is a water-soluble, blue-fluorescent dye that often used in multi-color applications, including flow cytometry and super-resolution microscopy using STORM. Its excitation is ideally suited for the 407 nm spectral line of the krypton laser or the 408 nm violet laser diode. AFDye™ 405 is structurally and spectrally similar to DyLight® 405, Alexa Fluor® 405, CF® 405S Dye, or any other pyrene-based fluorescent dyes.

AFDye™ 405 DBCO reacts with azides via a copper-free click chemistry reaction to form a stable triazole and does not require a Cu-catalyst or elevated temperatures. In applications where the presence of copper is a concern, AFDye™ 405 DBCO is an ideal alternative to fluorescent alkynes that require copper.

DyLight® and Alexa Fluor® are registered trademarks of Thermo Fisher Scientific. CF® Dye is registered trademark of Biotium Inc.

Azides, picolyl azides
Abs/Em Maxima
402/424 nm
Extinction coefficient
35,000 cm-1M-1
Water, DMSO, DMF
Spectrally similar dyes
Alexa Fluor® 405, DyLight® 405,
Molecular weight
774.79 (protonated), 1073.37 (triethylammonium salt)
Storage conditions
Shipping conditions
Ambient temperature


Alexa Fluor® is a registered trademark of Invitrogen, part of Thermo Fisher Scientific. Fluoroprobes is not affiliated with or endorsed by Thermo Fisher Scientific. AFDyes™ are GENERIC versions of Alexa Fluor® Dyes and ARE NOT manufactured, distributed, or affiliated in any way with Thermo Fisher Scientific.