AFDye 405 DBCO

Catalog#

Unit Size

Price (USD)

Availability

Qty

Cat.#: 1066-1

Unit size: 1 mg

$149.00

in stock

Cat.#: 1066-5

Unit size: 5 mg

$465.00

in stock

Cat.#: 1066-25

Unit size: 25 mg

$1,495.00

in stock

AFDye™ 405 is a water-soluble, blue-fluorescent dye that often used in multi-color applications, including flow cytometry and super-resolution microscopy using STORM. Its excitation is ideally suited for the 407 nm spectral line of the krypton laser or the 408 nm violet laser diode. AFDye™ 405 is structurally and spectrally similar to DyLight® 405, Alexa Fluor® 405, CF® 405S Dye, or any other pyrene-based fluorescent dyes.

AFDye™ 405 DBCO reacts with azides via a copper-free click chemistry reaction to form a stable triazole and does not require a Cu-catalyst or elevated temperatures. In applications where the presence of copper is a concern, AFDye™ 405 DBCO is an ideal alternative to fluorescent alkynes that require copper.

DyLight® and Alexa Fluor® are registered trademarks of Thermo Fisher Scientific. CF® Dye is registered trademark of Biotium Inc.

Specifications
Structure
Abs/Em Spectra
Documents

Reactivity

Azides, picolyl azides

Abs/Em Maxima

402/424 nm

Extinction coefficient

35,000 cm-1M-1

Solubility

Water, DMSO, DMF

Spectrally similar dyes

Alexa Fluor® 405, DyLight® 405,

Molecular weight

774.79 (protonated), 1073.37 (triethylammonium salt)

Storage conditions

-20C

Shipping conditions

Ambient temperature