AFDye 405 DBCO

Catalog#: 1066-1
Unit size: 1 mg
In stock
Catalog#: 1066-5
Unit size: 5 mg
In stock
Catalog#: 1066-25
Unit size: 25 mg
In stock
Catalog#: 1066-100
Unit size: 100 mg
In stock

AFDye™ 405 DBCO (Alexa Fluor 405 DIBO analog) reacts with azides via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. In application where the presence of copper is a concern AFDye™ 405 DBCO is an ideal alternative to copper requiring fluorescent alkynes.

AFDye™ 405 (Alexa Fluor® 405) is a bright, blue-fluorescent probe optimally excited by the 407 nm spectral line of the krypton laser or the 408 nm violet laser diode. This probe is water-soluble and its fluorescence is pH independent over a wide pH range. The brightness and photostability of this dye are best suited to direct imaging of moderate-abundance targets.

AFDye™ 405 dye is structurally identical to Invitrogen’s Alexa Fluor® 405

Azide, picolyl azide
Abs/Em Maxima
402/424 nm
Extinction coefficient
35,000 cm-1M-1
Water, DMSO, DMF
Spectrally similar dyes
Alexa Fluor® 405, DyLight® 405,
Molecular weight
Storage conditions
Shipping conditions
Ambient temperature


Alexa Fluor® Dyes is a registered trademark of Invitrogen, part of Thermo Fisher Scientific. Fluoroprobes is not affiliated with or endorsed by Thermo Fisher Scientific. AFDyes are GENERIC versions of Alexa Fluor® Dyes and ARE NOT manufactures, distributed, or affiliated in any way with Thermo Fisher Scientific.