AF 405 Cadaverine (Alexa Fluor 405 Cadaverine) is a carbonyl reactive building block used to modify carboxylic groups in the presence of activators (e.g. EDC, or DCC) or activated esters (e.g. NHS esters) through a stable amide bond. Another common application for AFDye™ 405 Cadaverine is cell fixing by treatment with formaldehyde or glutaraldehyde. It also often used as a water-soluble.
AFDye™ 405 (Alexa Fluor® 405 equivalent) is a water-soluble, blue-fluorescent dye that often used in multicolor applications, including flow cytometry and super-resolution microscopy using STORM. It’s excitation ideally suited for the 407 nm spectral line of the krypton laser or the 408 nm violet laser diode. AFDye™ 405 conjugates are pH-insensitive from pH 4 to pH10.
AFDye™ 405 dye is structurally identical to Invitrogen’s Alexa Fluor® 405