AFDye 405 Cadaverine (Alexa Fluor 405 Cadaverine equivalent) is a carbonyl reactive building block used to modify carboxylic groups in the presence of activators (e.g. EDC, or DCC) or activated esters (e.g. NHS esters) through a stable amide bond. Another common application for AFDye™ 405 Cadaverine is cell fixing by treatment with formaldehyde or glutaraldehyde. It also often used as a water-soluble.
AFDye™ 405 is a water-soluble, blue-fluorescent dye that often used in multicolor applications, including flow cytometry and super-resolution microscopy using STORM. It’s excitation ideally suited for the 407 nm spectral line of the krypton laser or the 408 nm violet laser diode. AFDye™ 405 is structurally and spectrally similar to DyLight® 405, Alexa Fluor® 405, CF® 405s Dye, or any other pyrene-based fluorescent dyes.
DyLight® and Alexa Fluor® are registered trademarks of Thermo Fisher Scientific. CF® Dye is registered trademark of Biotium Inc.