AFDye 405 Azide

Catalog#: 1064-1
Unit size: 1 mg
$129.00
In stock
Catalog#: 1064-5
Unit size: 5 mg
$395.00
In stock
Catalog#: 1064-25
Unit size: 25 mg
$1,295.00
In stock

AFDye™ 405 Azide is a water-soluble, blue-fluorescent dye that often used in multi-color applications, including flow cytometry and super-resolution microscopy using STORM. Its excitation is ideally suited for the 407 nm spectral line of the krypton laser or the 408 nm violet laser diode. AFDye™ 405 is structurally and spectrally similar to DyLight® 405, Alexa Fluor® 405, CF® 405S Dye, or any other pyrene-based fluorescent dyes.

AFDye™ 405 Azide can be reacted with terminal alkynes via a copper-catalyzed click reaction (CuAAC). It also reacts with strained cyclooctyne via a copper-free click chemistry reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures.

DyLight® and Alexa Fluor® are registered trademarks of Thermo Fisher Scientific. CF® Dye is registered trademark of Biotium Inc.

Reactivity
Alkynes, cyclooctynes
Abs/Em Maxima
402/424 nm
Extinction coefficient
35,000 cm-1M-1
Solubility
Water, DMSO, DMF
Spectrally similar dyes
Alexa Fluor® 405, DyLight® 405,
Molecular weight
640.65
Storage conditions
-20C
Shipping conditions
Ambient temperature

 

Alexa Fluor® is a registered trademark of Invitrogen, part of Thermo Fisher Scientific. Fluoroprobes is not affiliated with or endorsed by Thermo Fisher Scientific. AFDyes™ are GENERIC versions of Alexa Fluor® Dyes and ARE NOT manufactured, distributed, or affiliated in any way with Thermo Fisher Scientific.