AFDye 405 Alkyne

Catalog#

Unit Size

Price (USD)

Availability

Qty

Cat.#: 1067-1

Unit size: 1 mg

$139.00

in stock

Cat.#: 1067-5

Unit size: 5 mg

$445.00

in stock

Cat.#: 1067-25

Unit size: 25 mg

$1,395.00

in stock

AFDye™ 405 is a water-soluble, blue-fluorescent dye that often used in multi-color applications, including flow cytometry and super-resolution microscopy using STORM. Its excitation is ideally suited for the 407 nm spectral line of the krypton laser or the 408 nm violet laser diode. AFDye™ 405 is structurally and spectrally similar to DyLight® 405, Alexa Fluor® 405, CF® 405S Dye, or any other pyrene-based fluorescent dyes.

AFDye™ 405 Alkyne reacts with azides via a copper-catalyzed click reaction (CuAAC) to form a stable triazole linker. A probe for copper-less detection (AFDye™ 405 DBCO) is also available for application where the presence of copper is not acceptable.

DyLight® and Alexa Fluor® are registered trademarks of Thermo Fisher Scientific. CF® Dye is a registered trademark of Biotium Inc.

Specifications
Structure
Abs/Em Spectra
Documents

Reactivity

Azides, picolyl azides

Abs/Em Maxima

402/424 nm

Extinction coefficient

35,000 cm-1M-1

Solubility

Water, DMSO, DMF

Spectrally similar dyes

Alexa Fluor® 405, DyLight® 405, CF® 405s

Molecular weight

553.53 (protonated) 857.11 (triethylammonium salt)

Storage conditions

-20C

Shipping conditions

Ambient temperature