AFDye™ 405 Alkyne reacts with azides via a copper-catalyzed click reaction (CuAAC) to form a stable triazole linker. A probe for copper-less detection (AFDye™ 405 DBCO) is also available for application where the presence of copper is not acceptable.
AFDye™ 405 is a water-soluble, blue-fluorescent dye that often used in multicolor applications, including flow cytometry and super-resolution microscopy using STORM. It’s excitation ideally suited for the 407 nm spectral line of the krypton laser or the 408 nm violet laser diode. AFDye™ 405 is structurally and spectrally similar to DyLight® 405, Alexa Fluor® 405, CF® 405s Dye, or any other pyrene-based fluorescent dyes.
DyLight® and Alexa Fluor® are registered trademarks of Thermo Fisher Scientific. CF® Dye is registered trademark of Biotium Inc.