AFDye 405 Alkyne

Catalog#: 1067-1
Unit size: 1 mg
In stock
Catalog#: 1067-5
Unit size: 5 mg
In stock
Catalog#: 1067-25
Unit size: 25 mg
In stock
Catalog#: 1067-100
Unit size: 100 mg
In stock

AF 405 Alkyne (Alexa Fluor 405 Alkyne analog) is a water-soluble, bright, blue-fluorescent dye with excitation ideally suited for the 407 nm spectral line of the krypton laser or the 408 nm violet laser diode. AFDye™ 405 Alkyne probe is routinely used for imaging of azide-containing biomolecules.

AFDye™ 405 Alkyne reacts with azides via a copper-catalyzed click reaction (CuAAC) to form a stable triazole linker. A probe for copper-less detection (AFDye™ 405 DBCO) is also available for application where the presence of copper is not acceptable.

AFDye™ 405 dye is structurally identical to Invitrogen’s Alexa Fluor® 405

Azide, picolyl azide
Abs/Em Maxima
402/424 nm
Extinction coefficient
35,000 cm-1M-1
Water, DMSO, DMF
Spectrally similar dyes
Alexa Fluor® 405, DyLight® 405,
Molecular weight
Storage conditions
Shipping conditions
Ambient temperature


Alexa Fluor® Dyes is a registered trademark of Invitrogen, part of Thermo Fisher Scientific. Fluoroprobes is not affiliated with or endorsed by Thermo Fisher Scientific. AFDyes are GENERIC versions of Alexa Fluor® Dyes and ARE NOT manufactures, distributed, or affiliated in any way with Thermo Fisher Scientific.