AF 405 Acid (Alexa Fluor 405 Acid) is a water-soluble, blue-fluorescent dye that often used in multicolor applications, including flow cytometry and super-resolution microscopy using STORM. It’s excitation ideally suited for the 407 nm spectral line of the krypton laser or the 408 nm violet laser diode. AFDye™ 405 conjugates are pH-insensitive from pH 4 to pH10.
The carboxylic acid of AFDye™ 405 dye is a reagent of choice for the preparation of custom activated esters that often are not commercially available. Examples of such activated esters include sulfo-NHS, TFP (2,3,5,6-Tetrafluorophenol), STP (4-Sulfo-2,3,5,6-Tetrafluorophenol, Sodium Salt). Another common application for non-activated carboxylic acid is peptide modification during solid phase synthesis, which usually requires in-situ activation with peptide coupling regents, for example HATU. AFDye™ 405 Acid is also often used for control experiments, and for calibration.
AFDye™ 405 dye is structurally identical to Invitrogen’s Alexa Fluor® 405