AFDye 405 Acid

Catalog#: 1060-5
Unit size: 5 mg
In stock
Catalog#: 1060-25
Unit size: 25 mg
In stock
Catalog#: 1060-100
Unit size: 100 mg
In stock

AF 405 Acid (Alexa Fluor 405 Acid) is a water-soluble, blue-fluorescent dye that often used in multicolor applications, including flow cytometry and super-resolution microscopy using STORM. It’s excitation ideally suited for the 407 nm spectral line of the krypton laser or the 408 nm violet laser diode. AFDye™ 405 conjugates are pH-insensitive from pH 4 to pH10.

The carboxylic acid of AFDye™ 405 dye is a reagent of choice for the preparation of custom activated esters that often are not commercially available. Examples of such activated esters include sulfo-NHS, TFP (2,3,5,6-Tetrafluorophenol), STP (4-Sulfo-2,3,5,6-Tetrafluorophenol, Sodium Salt). Another common application for non-activated carboxylic acid is peptide modification during solid phase synthesis, which usually requires in-situ activation with peptide coupling regents, for example HATU. AFDye™ 405 Acid is also often used for control experiments, and for calibration.

AFDye™ 405 dye is structurally identical to Invitrogen’s Alexa Fluor® 405

Primary amines (requires activation)
Abs/Em Maxima
402/424 nm
Extinction coefficient
35,000 cm-1M-1
Water, DMSO, DMF
Spectrally similar dyes
Alexa Fluor® 405, DyLight® 405, Cascade Blue
Molecular weight
Storage conditions
Shipping conditions
Ambient temperature


Alexa Fluor® Dyes is a registered trademark of Invitrogen, part of Thermo Fisher Scientific. Fluoroprobes is not affiliated with or endorsed by Thermo Fisher Scientific. AFDyes are GENERIC versions of Alexa Fluor® Dyes and ARE NOT manufactures, distributed, or affiliated in any way with Thermo Fisher Scientific.