AFDye 350 Azide

Catalog#: 1004-1
Unit size: 1 mg
In stock
Catalog#: 1004-5
Unit size: 5 mg
In stock
Catalog#: 1004-25
Unit size: 25 mg
In stock
Catalog#: 1004-100
Unit size: 100 mg
In stock

AFDye™ 350 Azide (Alexa Fluor® 350 equivalent) is a blue-fluorescent azide-activated probe that reacts with terminal alkynes via a copper-catalyzed click reaction (CuAAC). It also reacts with strained cyclooctyne via a copper-free click chemistry reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures.

AFDye™ 350 is a water-soluble moderately photostable, blue-fluorescent probe optimally excited by the 350 nm laser line. It is routinely used for generation of stable signal in imaging and flow cytometry. The brightness and photostability of blue dyes are best suited to direct imaging of high-abundance targets.

AFDye™ 350 dye structurally is identical to Invitrogen’s Alexa Fluor® 350

Alkynes, cyclooctynes
Abs/Em Maxima
346/445 nm
Extinction coefficient
19,000 cm-1M-1
Water, DMSO, DMF
Spectrally similar dyes
Alexa Fluor® 350, AMCA, DyLight® 350
Molecular weight
Storage conditions
Shipping conditions
Ambient temperature

Alexa Fluor® Dyes is a registered trademark of Invitrogen, part of Thermo Fisher Scientific. Fluoroprobes is not affiliated with or endorsed by Thermo Fisher Scientific. AF Dyes are GENERIC versions of Alexa Fluor® Dyes and ARE NOT manufactures, distributed, or affiliated in any way with Thermo Fisher Scientific.