AFDye™ 350 Azide (Alexa Fluor® 350 Azide equivalent) is a blue-fluorescent, azide-activated probe that reacts with terminal alkynes via a copper-catalyzed click reaction (CuAAC). It also reacts with strained cyclooctyne via a copper-free click chemistry reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures.
AFDye™ 350 is a water-soluble, moderately photostable, blue-fluorescent probe optimally excited by the 350 nm laser line. It is routinely used for generation of stable signal in imaging and flow cytometry. The brightness and photostability of blue dyes are best suited to direct imaging of high-abundance targets.
AFDye™ 350 dye is structurally identical to Invitrogen’s Alexa Fluor® 350 Dye.