AFDye™ 350 Alkyne (Alexa Fluor® 350 Alkyne equivalent) is a blue-fluorescent, alkyne-activated probe routinely used for imaging azide-tagged biomolecules. AFDye™ 350 Alkyne reacts with azides via a copper-catalyzed click reaction (CuAAC) to form a stable triazole linker.
AFDye™ 350 is a water-soluble, moderately photostable, blue-fluorescent probe optimally excited by the 350 nm laser line. It is routinely used for generation of stable signal in imaging and flow cytometry. The brightness and photostability of blue dyes are best suited to direct imaging of high-abundance targets.
AFDye™ 350 dye structurally is identical to Invitrogen’s Alexa Fluor® 350 Dye.