AFDye 350 Acid

Catalog#: 1003-1
Unit size: 1 mg
$95.00
In stock
Catalog#: 1003-5
Unit size: 5 mg
$295.00
In stock
Catalog#: 1003-25
Unit size: 25 mg
$895.00
In stock
Catalog#: 1003-100
Unit size: 100 mg
$1,895.00
In stock

AFDye™ 350 Acid (Alexa Fluor® 350 Acid) is an amine reactive, non-activated, water-soluble, blue-emitting dye used for derivatizing amine-containing molecules in the presence of activators (e.g. EDC or HATU) through a stable, covalent amide bond. The NHS ester (or succinimidyl ester) of this dye is also available for conjugating AFDye™ 350 to the primary amines of proteins or antibodies (Lys), amine-modified oligonucleotides, and other amine-containing molecules.

AFDye™ 350 is a moderately photostable, blue-fluorescent probe optimally excited by the 350 nm laser line routinely used for producing bright, blue fluorescent conjugates for imaging and flow cytometry. The emission of AFDye™ 350 conjugates is slightly shorter than that of AMCA or AMCA conjugates, reducing AFDye™ 350’s spectral overlap with the emission of fluorescein or Alexa Fluor® 488 dye.

AFDye™ 350 dye structurally is identical to Invitrogen’s Alexa Fluor® Dye 350.

Reactivity
Primary amines (needs activation)
Abs/Em Maxima
346/445 nm
Extinction coefficient
19,000 cm-1M-1
Solubility
Water, DMSO, DMF
Spectrally similar dyes
Alexa Fluor® 350, AMCA, DyLight® 350
Molecular weight
313.27
Storage conditions
-20C
Shipping conditions
Ambient temperature

Alexa Fluor® is a registered trademark of Invitrogen, part of Thermo Fisher Scientific. Fluoroprobes is not affiliated with or endorsed by Thermo Fisher Scientific. AFDyes™ are GENERIC versions of Alexa Fluor® Dyes and ARE NOT manufactured, distributed, or affiliated in any way with Thermo Fisher Scientific.