6,8-Difluoro-7-hydroxy-4-methylcoumarin NHS Ester

Catalog#: 1242-5
Unit size: 5 mg
$95.00
In stock
Catalog#: 1242-25
Unit size: 25 mg
$195.00
In stock
Catalog#: 1242-100
Unit size: 100 mg
$595.00
In stock

6,8-Difluoro-7-hydroxy-4-methylcoumarin NHS Ester (Marina Blue® NHS Ester) exhibit bright blue fluorescence emission near 460 nm is optimally excited by the intense 365 nm spectra line of the mercury-arc lamp and detect optimally with DAPI optical filter sets. Because the pKa value of 6,8-Difluoro-7-hydroxy-4-methylcoumarin derivatives are 2-3 log units lower compared to those of the corresponding 7-hydroxycoumarin conjugates, 6,8-Difluoro-7-hydroxy-4-methylcoumarin conjugates are strongly fluorescent even at neutral pH.

6,8-Difluoro-7-hydroxy-4-methylcoumarin NHS Ester reacts specifically and efficiently with a primary amine (e.g., side chain of lysine residues or aminosilane-coated surfaces) at pH 7-9 to form a stable, covalent amide bond. The NHS ester (or succinimidyl ester) is the most popular tool for conjugating dyes to the primary amines of protein or antibody (Lys), amine-modified oligonucleotides, and other amine-containing molecules.

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Reactivity
Primary amines
Abs/Em Maxima
365/460 nm
Extinction coefficient
19,000 cm-1M-1
Solubility
DMSO, DMF
Spectrally similar dyes
Alexa Fluor® 350, AMCA, DyLight® 350
Molecular weight
367.26
Storage conditions
-20C
Shipping conditions
Ambient temperature

Marina Blue® Dyes is a registered trademark of Invitrogen, part of Thermo Fisher Scientific. Fluoroprobes is not affiliated with or endorsed by Thermo Fisher Scientific.