5(6)-TAMRA NHS Ester

Catalog#: 1254-25
Unit size: 25 mg
In stock
Catalog#: 1254-100
Unit size: 100 mg
In stock
Catalog#: 1254-1000
Unit size: 1000 mg
In stock

5(6)-TAMRA NHS Ester (5(6)-Carboxytetramethylrhodamine, TMR, TRITC) is a bright orange-fluorescent dye with excitation ideally suited to the 532 nm or 546 nm laser lines. It has been used widely for preparing peptide, protein, nucleotide and nucleic acid conjugates, especially fluorescent antibodies and avidin derivatives used in immunochemistry. The absorbance and emission maxima of TAMRA conjugates are 553 nm and 575 nm respectively.

TAMRA NHS Ester reacts specifically and efficiently with a primary amine (e.g., side chain of lysine residues or aminosilane-coated surfaces) at pH 7-9 to form a stable, covalent amide bond. The NHS ester (or succinimidyl ester) is the most popular tool for conjugating dyes to the primary amines of protein or antibody (Lys), amine-modified oligonucleotides, and other amine-containing molecules.

Primary amine
Abs/Em Maxima
553/575 nm
Extinction coefficient
92,000 cm-1M-1
Spectrally similar dyes
Alexa Fluor® 546, TAMRA, CF™ 543, MB™ 543
Molecular weight
Storage conditions
Shipping conditions
Ambient temperature

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