5-TAMRA NHS Ester

Catalog#: 1255-25
Unit size: 25 mg
$129.00
In stock
Catalog#: 1255-100
Unit size: 100 mg
$395.00
In stock
Catalog#: 1255-1000
Unit size: 1000 mg
$1,295.00
In stock

Although the mixed isomers of 5(6)-TAMRA Acid is a preferred, routinely used orange-fluorescent dye for staining proteins, it is rearly used for labeling peptides and nucleotides. Purification of 5(6)-TAMRA labeled peptide and nucleotides might be troublesome due to significant signal broadening in HPLC purification. Peptides and nucleotides labeled with a single isomer TAMRA usually give better resolution in HPLC purification that is often required in the conjugation processes. 5-TAMRA is more often used for labeling peptides and proteins. 6-TAMRA is predominately used for labeling nucleotides and sequencing nucleic acids. 5-TAMRA dye is a bright fluorescent label is compatible with various excitation sources including mercury arc, tungsten and xenon arc lamps, the 544 nm line of the Helium-Neon laser and the 532 nm green laser line.

The NHS Ester reacts specifically and efficiently with a primary amine (e.g., side chain of lysine residues or aminosilane-coated surfaces) at pH 7-9 to form a stable, covalent amide bond. The NHS ester (or succinimidyl ester) is the most popular tool for conjugating dyes to the primary amines of protein or antibody (Lys), amine-modified oligonucleotides, and other amine-containing molecules.

Reactivity
Primary amine
Abs/Em Maxima
553/575 nm
Extinction coefficient
92,000 cm-1M-1
Solubility
DMSO, DMF, MeOH
Spectrally similar dyes
Alexa Fluor® 546, TAMRA, CF™ 543, MB™ 543
Molecular weight
527.53
Storage conditions
-20C
Shipping conditions
Ambient temperature

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